PERENDAMAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) UNTUK MENINGKATKAN IMUNITAS DAN SINTASAN UDANG VANAME (Litopenaeus vannamei) YANG TERINFEKSI BAKTERI Vibrio parahaemolyticus
Soaking In Jeruju Leaf Extract (Acanthus ilicifolius) To Enhance Immunity And Survival Rates In Vaname Shrimp (Litopenaeus vannamei) Infected With The Bacterium Vibrio parahaemolyticus
DOI:
https://doi.org/10.33096/wgsj4s87Kata Kunci:
Acanthus ilicifolius; White shrimp; Vibrio parahaemolyticus; Imunostimulan; Survival rate.Abstrak
White shrimp (Litopenaeus vannamei) is a leading aquaculture commodity in Indonesia whose production is often hampered by Vibrio parahaemolyticus infection, the causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND) with mortality reaching 85–100%. This study aimed to determine the effect of immersion in jeruju leaf extract (Acanthus ilicifolius) on the immune response and survival of V. parahaemolyticus-infected white shrimp, and to identify its optimal concentration. A Completely Randomized Design (CRD) was used with five groups: negative control, positive control, and extract immersion at 200, 300, and 400 mg/L for 30 minutes. Data were analyzed using One Way ANOVA followed by Duncan's test. Results showed that jeruju leaf extract immersion significantly affected (p<0.05) THC, DHC composition (an increase in the proportion of granular cells), and post-infection survival rate. The 400 mg/L concentration (treatment C) produced the best results, with the highest THC of 15.08 × 10⁶ cells/ml, the highest proportion of granular cells at 65.67%, and the highest SR of 79.17%, compared to only 48.33% in the positive control. These findings demonstrate that jeruju leaf extract has potential as an environmentally friendly herbal immunostimulant for controlling V. parahaemolyticus infection in white shrimp aquaculture.
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