PERBANDINGAN IMUNOSTIMULAN YANG BERBEDA TERHADAP GAMBARAN DARAH UDANG VANAME (Litopenaeus vannamei) DI TAMBAK PENDAMPINGAN PT SURI TANI PEMUKA

Safira Surya Himzanah; Mad Rudi; Himawan Prasetiyo; Narendra Santika Hartana

Safira Surya Himzanah Universitas Pendidikan Indonesia
Mad Rudi Universitas Pendidikan Indonesia
Himawan Prasetiyo Institut Pertanian Bogor
Narendra Santika Hartana PT Suri Tani Pemuka, Wisma Millenia

Keywords: udang vaname, imunostimulan, THC, DHC

Abstract

Vaname shrimp (Litopenaeus vannamei) is one of the main commodities in the field of Indonesian fisheries. Effort to overcome the disease, one of which is done by giving immunostimulants to the feed. Immunostimulants can be used include Lactobacillus and Saccharomyces (treatment A) and selenium and β-glucan (treatment B). The purpose of this study is to determine the effectiveness of immunostimulants based on non-specific immune systems and water quality parameters such as salinity. This research design uses experimental with a randomized group design on 2 plots of 3 replicates in PT Suri Tani Pemuka (STP) assistance pond. It uses 2 factors, namely salinity, maintenance, and different types of immunostimulants. The parameters measured are blood images of total hemocyte count (THC), differential hemocyte count (DHC), and maintenance water quality. The results of THC observations showed that the administration of Lactobacillus and Saccharomyces in the second week of observation gave real different results (P<0.05) with an average value of 8.65x10⁶ cel / ml. DHC results with administration of Lactobacillus and Saccharomyces as well as selenium and β-glucan gave significantly different results (P<0.05), meaning that immunostimulant administration can affect the amount of granular and hyaline in vaname shrimp hemolymph. Administration of Lactobacillus and Saccharomyces as well as selenium and β-glucan can increase 2-8% and 3-4% respectively against granular amounts. The maintenance salinity factor gives no real difference (P>0.05) to THC and DHC values. Giving Lactobacillus and Saccharomyces can be an alternative in increasing immunity in vaname shrimp rearing with a salinity of 2-7 ppt.

References

[BPS] Badan Pusat Statistik. (2022a). Produksi Perikanan Budidaya di Indonesia. Jakarta: Badan Pusat Statistik.

[BPS] Badan Pusat Statistik. (2022b). Produksi Perikanan Budidaya Menurut Komoditas Utama. Jakarta: Badan Pusat Statistik.

Ammas, S. (2013). Analisis Peningkatan Haemosit Post Larva Udang Vannamei (Litopenaeus Vannamei Linnaeus) Pasca Perendaman Ekstrak Ragi Roti (Saccharomyces Cerevisiae) Pada Konsentrasi Berbeda Terhadap Bakteri Vibrio Harveyi [Skripsi].

Amri, K. (2013). Budidaya Udang Vaname. Gramedia Pustaka Utama.

Andrade, A. J. (2011). Shrimp Immunological Reactions Againts WSSV: Role of Haemocytes on WSSV Fate.

Andriani, Y., Kanza, A. A., Rustama, M. M., & Safitri, R. (2017). Karakterisasi Bacillus dan Lactobacillus yang dienkapsulasi dalam Berbagai Bahan Pembawa untuk Probiotik Vannamei (Litopenaeus vannamei Boone, 1931). Journal Perikanan Dan Kelautan, 7(2), 142–154.

Arfiati, D., Nuriyani, & Kharismayanti, H. F. (2018). Crassostrea : Tiram Bakau dan Tiram Batu. UB Press.

Asrin, Sidik, A. S., & Sukarti, K. (2020). Pengaruh Salinitas Terhadap Pertumbuhan dan Frekuensi Udang Bintik (Metapenaeus affinis). Aquawarman: Jurnal Sains Dan Teknologi Akuakultur, 6(1), 113–120.

Cao, L. (2012). Farming Shrimp For The Future: A Sustainability Analysis of Shrimp Farming In China. University of Michigan.

Darwantin, K., Sidik, R., & Mahasri, G. (2016). Efisiensi Penggunaan Imunostimulan dalam Pakan Terhadap Laju Pertumbuhan, Respon Imun dan Kelulushidupan Udang Vannamei (Litopenaeus vannamei). Jurnal Biosains Pascasarjana, 18(2), 123–140.

Ekasari, J., Napitupulu, J. L. F., & Surawidjaja, E. H. (2016). Imunitas dan Pertumbuhan Udang Galah yang diberi Pakan dengan Suplementasi β-glukan. Jurnal Akuakultur Indonesia, 15(1), 41–48.

Ekawati, A. W., Nursyam, H., Widjayanto, E., & Marsoedi, M. (2012). Diatomae Chaetoceros ceratosporum dalam Formula Pakan Meningkatkan Respon Imun Seluler Udang Windu (Penaeus monodon Fab.). The Journal of Experimental Life Science, 2(1), 20–28.

Grag, S. (2007). Effect of oral administration of 1-thyroxine (T4) on growth performance, digestibility, and nutrient retention in Channa punctatus (Bloch) and Heteropneustes fossilis (Bloch). Fish Physiology and Biochemistry, 33, 347–358.

Gonzalez, J. V. (2013). Efecto de la Modulacion Inmunologica en la Supervivencia del Cultivo de Camaron Litopenaeus vannamei. Tesis. Ekuador: Universidad de Guayaquil.

Jannah, M., Junaidi, M., Setyowati, D. N., & Azhar, F. (2018). Pengaruh Pemberian Lactobacillus sp dengan Dosis yang Berbeda Terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) yang diinfeksi Bakteri Vibrio parahaemolyticus. Jurnal Kelautan, 11(2), 140–151.

Kurniawan, M. H., Putri, B., & Elisdiana, Y. (2018). Efektivitas Pemberian Bakteri Bacillus polymyxa Melalui Pakan Terhadap Imunitas Non Spesifik Udang Vannamei (Litopenaeus vannamei). Jurnal Rekayasa dan Teknologi Budidaya Perairan, 7(1), 739–750.

Lee, M. H., & Shiau, S. Y. (2004). Vitamin E Requirements of Juvenile Grass Shrimp, Penaeus monodon and Effects on Nonspecific Immune Responses. Fish & Shellfish Immunology, 16, 475–485.

Manoppo, H., & Kolopita, Magdalena. E. F. (2014). Respon Imun Krustase. Budidaya Perairan, 2(2), 22–26.

Rachmawati, D., Hutabarat, J., Susilowati, T., Samidjan, I., & Pranggono, H. (2020). Penambahan Saccharomyces cerevisiae pada Pakan Buatan Komersial Benih Lele Sangkuriang (Clarias gariepinus Var. Sangkuriang) Terhadap Efisiensi Pemanfaatan Pakan, Peetumbuhan, dan Kelulushidupan. PENA Akuatika, 19(2), 28–38.

Ridlo, A., & Pramesti, R. (2014). Aplikasi Ekstrak Rumput Laut sebagai Agen Imunostimulan Sistem Pertahanan Non Spesifik pada Udang Vannamei (Litopennaeus vannamei). Ilmu Kelautan, 14(3), 133–137.

Rosmawaty, R., Rosidah., & Liviawaty, E. (2016). Pemanfaatan Ekstrak Kulit Jengkol dalam Pakan Ikan Untuk Meningkatkan Imunitas Benih Gurame (Osphronemus gouramy) terhadap Infeksi Bakteri Aeromonas hydrophila. Jurnal Perikanan Kelautan. 7(1): 14– 22.

Sabban, I. (2014). Mekanisme Respon Imun Pada Udang. Kediri: Institut Ilmu Kesehatan Bhakti Wiyata.

Sakai, M. (1999). Current Research Status of Fish Immunostimulants. Aquaculture, 172, 63–92.

Subaidah, S., Sofiati, Manijo, & Titis. (2018). Penambahan Nukleotida dalam Pakan Pembesaran sebagai Immunostimulan pada Udang Vaname, Litopenaeus vannamei.

Syadillah, A., Hilyana, S., & Marzuki, M. (2020). Pengaruh Penambahan Bakteri (Lactobacillus sp) dengan Konsentrasi Berbeda Terhadap Pertumbuhan Udang Vannamei (Litopenaeus vannamei). Jurnal Perikanan, 10(1), 8–19.

Tampangallo, B. R., Pakidi, C. S., & Rantetondok, A. (2012). Respon Imun Udang Windu (Panaeus monodon) yang dipapar bakteri Vibrio harveyi. Prosiding InSINas.

Vernberg, W. B., & Vernberg, F. J. (1972). Environmental Physiology of Marine Animal. Springer-Verlag.

Widodo, W., Ilmiah, & Hadijah, S. (2022). Status Penyakit Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) yang Menginfeksi Budidaya Udang Vannamei (Litopenaeus vannamei) di Kabupaten Pinrang. Journal of Indonesian Tropical Fisheries, 5(2), 216–227.

Yudiati, E., Sedjati, S., Enggar, I., & Hasibuan, I. (2009). Dampak Pemaparan Logam Berat Kadmiun pada Salinitas yang Berbeda Terhadap Mortalitas dan Kerusakan Jaringan Insang Juvenil Udang Vaname (Litopenaeus vannamei). Indonesian Journal of Marine Science, 14(4), 29–35.